Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry. (October 2018)

• Record Type: Journal Article
• Authors: Osbak, Kara K; Van Raemdonck, Geert A; Dom, Martin; Cameron, Caroline E; Meehan, Conor J; Deforce, Dieter; Ostade, Xaveer Van; Kenyon, Chris R; Dhaenens, Maarten
• Is Part Of: Future microbiology. Volume 13:Number 13(2018)
• Page Start: 1497
• Page End: 1510
• Publication Date: 2018-10
• Subjects: antigen test -- biomarker discovery -- data-independent acquisition -- mass spectrometry -- proteomics -- syphilis -- Treponema pallidum -- urine; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0182
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis.Materials & methods: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers.Results & conclusion: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.