A Genome-Wide Screen for Sporulation-Defective Mutants in Schizosaccharomyces pombe. Issue 6 (1st June 2014)

• Record Type: Journal Article
• Authors: Ucisik-Akkaya, Esma; Leatherwood, Janet K; Neiman, Aaron M
• Is Part Of: G3. Volume 4:Issue 6(2014)
• Page Start: 1173
• Page End: 1182
• Publication Date: 2014-06-01
• Subjects: knockout collection -- erp2 -- erp5 -- forespore membrane; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.114.011049
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: Yeast sporulation is a highly regulated developmental program by which diploid cells generate haploid gametes, termed spores. To better define the genetic pathways regulating sporulation, a systematic screen of the set of ~3300 nonessential Schizosaccharomyces pombe gene deletion mutants was performed to identify genes required for spore formation. A high-throughput genetic method was used to introduce each mutant into an h 90 background, and iodine staining was used to identify sporulation-defective mutants. The screen identified 34 genes whose deletion reduces sporulation, including 15 that are defective in forespore membrane morphogenesis. In S. pombe, the total number of sporulation-defective mutants is a significantly smaller fraction of coding genes than in S. cerevisiae, which reflects the different evolutionary histories and biology of the two yeasts.