Structure and gene cluster of the O-antigen of Enterobacter cloacae G2559. (September 2022)

• Record Type: Journal Article
• Authors: Wang, Min; Filatov, Andrei V.; Shashkov, Alexander S.; Liu, Jiafei; Perepelov, Andrei V.
• Is Part Of: Carbohydrate research. Volume 519(2022)
• Publication Date: 2022-09
• Subjects: Enterobacter cloacae -- Bacterial polysaccharide structure -- O-antigen -- Lipopolysaccharide -- O-antigen gene cluster; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2022.108612
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2559 and studied by sugar analysis along with 1D and 2D 1 H and 13 C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established. Image 1 The O-antigen gene cluster of Enterobacter cloacae G2559 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-antigen structure. Graphical abstract: Image 1 Highlights: E. cloacae is the important multi-drug resistant bacteria. The O-specific polysaccharide structure of E. cloacae G2559 was established. New OPS is consists of a number of pentasaccharide repeating O-units. The O-antigen gene cluster of E. cloacae G2559 was sequenced and annotated.