Structural and functional characteristics of the hippocampus in depressive-like recipients after transplantation of in vitro caffeine-modulated immune cells. (24th August 2022)

• Record Type: Journal Article
• Authors: Markova, Evgeniya V.; Knyazheva, Maria A.; Tikhonova, Maria A.; Amstislavskaya, Tamara G.
• Is Part Of: Neuroscience letters. Volume 786(2022)
• Publication Date: 2022-08-24
• Subjects: A2A adenosine receptors of type 2A -- AOI area of interest -- BDNF brain-derived neurotrophic factor -- CA cornu Ammonis of the hippocampus -- INF-γ interferon gamma -- IL-1β interleukin 1 beta -- IL-4 interleukin 4 -- IL-6 interleukin 6 -- IL-10 interleukin 10 -- IL-12 interleukin 12 -- TNF-α tumor necrosis factor alpha; Depression -- Modulated immune cells -- Mice -- Brain-derived neurotrophic factor -- Cytokines; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2022.136790
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: The immune cells were treated in vitro with caffeine. Modulated splenocytes were transplanted to depressive recipients from syngeneic mice. The transplantation increased neuronal density and BDNF levels in the hippocampus. Modulated immune cells decreased the hippocampal levels of pro-inflammatory cytokines. The transplantation augmented the hippocampal levels of anti-inflammatory cytokines. Abstract: The hippocampus is a key anatomical brain region associated with depression. On the other hand, immune cells and their releasing cytokines play an essential role in stress and depression. Noteworthy that the most of psychoactive drugs produce unidirectional effects on the cells of both nervous and immune systems. This suggests the immunotherapy for behavioral disorders based on the treatment with autologous immune cells in which functional activity was modulated ex vivo by a psychoactive drug. Here, we treated the immune cells of depressive-like mice in vitro with caffeine (100 μg per 15 × 10 6 cells). The effects of caffeine-treated immune cells transplantation on neuronal density, production of brain-derived neurotrophic factor (BDNF) and a number of cytokines in the hippocampus of depressive-like syngeneic animals were studied. In depressive-like recipients, an increase in the density of pyramidal neurons in CA1 and CA3 hippocampal regions, accompanied with augmented level of BDNF, decreased levels of pro-inflammatory (IL-1β, IL-6, INF-γ, and TNF-α) and increased levels of anti-inflammatory (IL-10 and IL-4) cytokines was found. The mechanisms of the revealed structural and functional alterations in the hippocampus of depressive-like recipients after transplantation of caffeine-treated immune cells are discussed.