SARS-CoV-2 antibody response after BNT162b2 mRNA vaccine in healthcare workers: Nine-month of follow-up. (August 2022)

• Record Type: Journal Article
• Authors: Mastroianni, Franco; Guida, Pietro; Bellanova, Grazia; Valentina De Nicolò, Edy; Righetti, Giulia; Formoso, Maurizio; Celani, Fabrizio
• Is Part Of: Vaccine. Volume 11(2022)
• Publication Date: 2022-08
• Subjects: COVID-19 -- Antibody responses -- Vaccine
• DOI: 10.1016/j.jvacx.2022.100175
• Languages: English
• ISSNs: 2590-1362

Abstract

Highlights: Pandemic coronavirus disease 2019 (COVID-19) has led to a worldwide vaccination. Post- vaccine antibody responsewas correlated with age. The reduction of antibodies response was more evidentat 12 than 4 weeks compared to 9 months. Abstract: We collected sequential serum samples (0, 4, 12 weeks, 9 months) for the determination of S-RDB IgG levels from 103 vaccinated healthy subjects (age 45 ± 13 years; 60 women), in order to evaluate neutralizing antibody response against SARS-CoV-2 in healthy healthcare workers (HCWs) after the administration of two doses of BNT162b2 SARS-CoV-2 mRNA vaccine. Every subject received two doses of mRNA vaccine BNT162b2 (Pfizer-BioNTech), 21 days apart (January-February 2021). Furthermore, antibody titer of 14 subjects who were hospitalized for symptomatic COVID-19 was evaluated. Antibody response was (median, interquartile range) 35 U/mL (10–104) at baseline, 1960 (1241–3221) at 4 weeks, 791 (388–1179) at 12 weeks and 524 (273–931) at 6 months. Antibody response was inversely correlated with age at all timepoints (p < 0.001) while gender and Body Mass Index had no significant effect. At multivariate analysis, post-baseline values were significantly higher than baseline (p < 0.001) with a reduction at 12 weeks and 9 months (p < 0.001). Antibody response of hospitalized subjects who did not receive vaccination, symptomatic for COVID 19 infection, was 103 (25–557) U/mL, significantly higher than baseline (p = 0.007) of study population but lower than all post-baseline determinations (p < 0.001). Younger subjects showed a stronger response and a lower decrease of antibody titers compared to the classes of older subjects. SARS-CoV2 infection was excluded by performing 1017 nasopharyngeal RT-PCR swabs on the study cohort. The second dose of mRNA vaccine resulted in an antibody response effective in preventing infection in a population of healthcare professionals. The antibody level was stable through week 12, showing a reduction in the following six months.