Accumulation of lipids by the oleaginous yeast Yarrowia lipolytica grown on carboxylic acids simulating syngas and carbon dioxide fermentation. (September 2022)

• Record Type: Journal Article
• Authors: Naveira-Pazos, Cecilia; Veiga, María C.; Kennes, Christian
• Is Part Of: Bioresource technology. Volume 360(2022)
• Publication Date: 2022-09
• Subjects: Biodiesel -- Carbon dioxide -- Lipids -- Volatile fatty acids -- Yarrowia lipolytica; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127649
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Lipid synthesis by oleaginous yeasts is a promising alternative to vegetable oils. Y. lipolytica can bioconvert mixed C 2 -C6 VFAs from gas fermentation into lipids. Yarrowia lipolytica shows preference for acetic acid over butyric and caproic acids. Y. lipolytica used the VFA mixture at a rate of 0.664 g/L·h with lipid accumulation. The lipid profile obtained shows its high suitability for biodiesel production. Abstract: Volatile fatty acids (VFAs) can be considered as low-cost carbon substrates for lipid accumulation by oleaginous yeasts. This study demonstrates that a common mixture of VFAs, typically obtained from the anaerobic fermentation of C1-gases by some acetogenic bacteria, can be used in a second aerobic fermentation with the yeast Yarrowia lipolytica to obtain lipids as precursors of biodiesel. In the batch experiments, the preference of Yarrowia lipolytica W29 for acetic acid over butyric and caproic acids was demonstrated, with the highest consumption rate reaching 0.664 g/L·h. In the bioreactor experiments, the amount initial biomass inoculated, as well as the initial acid concentration, were found to have a significant influence on the process. Though the lipid content was relatively low, it can be optimized and further improved. Oleic, linoleic and palmitic acids accounted for about 80 % of the fatty acids in the lipids, which makes them suitable for biodiesel.