The fate of antibiotic resistance genes, microbial community, and potential pathogens in the maricultural sediment by live seaweeds and oxytetracycline. (15th September 2022)

• Record Type: Journal Article
• Authors: Feng, Lijuan; Xiao, Changyan; Luo, Yuqin; Qiao, Yan; Chen, Dongzhi
• Is Part Of: Journal of environmental management. Volume 318(2022)
• Publication Date: 2022-09-15
• Subjects: Seaweed -- Antibiotic resistance genes (ARGs) -- Oxytetracycline -- Vibrio -- Tetracycline resistance genes; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2022.115597
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: Three common seaweeds including Ulva fasciata, Codium cylindricum and Ishige okamurai were used for the remediation of maricultural wastewater and sediment in the presence/absence of trace level of oxytetracycline (OTC) in lab-scale experiments. Higher NO3 − -N and PO4 3- -P removal rates were achieved due to the presence of seaweeds, and trace OTC also had a positive effect on NO3 − -N removal. A slight variation of 2.10–2.15% were observed in the total relative abundances of antibiotic resistance genes (ARGs) of different sediment samples after one-month operation. However, the variation of ARGs profiles by the co-existence of different seaweeds and OTC was in the descending order of Ishige okamurai > Codium cylindricum > Ulva fasciata, which was in accordance with the variation of microbial hosts at genus level. The abundance of dominant tetracycline resistance genes promoted by the co-existence of different seaweeds and OTC in compared with the presence of single seaweed or OTC via metagenomic sequencing and qPCR analysis, and the co-existence of Ishige okamurai and OTC exhibited the largest impact. The potential pathogens were more sensitive to the co-existence of seaweed and OTC than single seaweeds. Meanwhile, a variety of ARGs were enriched in the pathogens, and the dominant pathogenic bacteria of Vibrio had 133 Vibrio species with 28 subtypes of ARGs. The variation of ARGs profiles in the sediment were strongly related with the dominant phyla Proteobacteria, Actinobacteria, Firmicutes, Planctomycetes and Cyanobacteria. Besides, Nitrate level exhibited more significant effect on ∑ARGs, ARGs resistant to vancomycin and streptogramin _a, while phosphate level exhibited more positively significant effect on ARGs resistant to fosmidomycin, ATFBT and cephalosporin. Highlights: Seaweed and trace OTC significantly promoted nutrients removal. A slight variation of total ARGs in the sediment after one-month operation. Synergy effects of seaweed and OTC on ARGs, TRGs, microbial community and pathogens. ARGs profiles strongly related with the dominant phyla Proteobacteria, Actinobacteria, etc. Ishige okamurai and OTC exhibited the largest effect on ARGs and microbial community.