Evaluation of ion release and the recharge ability of glass-ionomer cement containing BioUnion filler using an in vitro saliva-drop setting assembly. Issue 5 (May 2021)

• Record Type: Journal Article
• Authors: Kohno, Tomoki; Liu, Yuhan; Tsuboi, Ririko; Kitagawa, Haruaki; Imazato, Satoshi
• Is Part Of: Dental materials. Volume 37:Issue 5(2021)
• Page Start: 882
• Page End: 893
• Publication Date: 2021-05
• Subjects: ANOVA analysis of variance -- CFU colony-forming units -- EPS extracellular polysaccharide -- GIC glass-ionomer cement -- HSD honest significant difference; Glass-ionomer cement -- Zinc -- Ion release -- Recharge -- Saliva -- Biofilm; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2021.02.022
• Languages: English
• ISSNs: 0109-5641

Abstract

Highlights: An in vitro saliva-drop setting was assembled to evaluate ion-release properties. GIC containing BioUnion filler releases Zn 2+ and F − under acidic conditions. Re-uptake of Zn 2+ and F − in the GIC could be obtained. Release of Zn 2+ from the GIC after recharging hinders biofilm formation. Abstract: Objective: A glass-ionomer cement (GIC) containing BioUnion filler has been reported to release Zn 2+ under acidic conditions and to inhibit oral bacteria on its surface. However, previous results are based on in vitro experiments under static conditions. This study aimed to assemble an in vitro saliva-drop setting to simulate in vivo conditions of the oral cavity and to investigate the ion releasing and recharging properties of the GIC containing BioUnion filler. Methods: The effective concentrations of Zn 2+ and F − against Streptococcus mutans and saliva-derived multi-species biofilms were determined. Artificial saliva was dropped on the GIC containing BioUnion filler using the in vitro saliva-drop setting assembly and was periodically replaced with acetic acid. Ion release/recharge properties were investigated by measuring the release concentrations of Zn 2+ and F − . Results: The concentration of Zn 2+ released from the BioUnion filler-containing GIC during seven days with repeated exposure to acid could be maintained at the level to inhibit S. mutans and saliva-derived multi-species biofilm formation. Moreover, the BioUnion filler-containing GIC could be recharged with Zn 2+ and F − by the application of a tooth gel containing Zn 2+ and F − . The release concentration of Zn 2+ after recharging was significantly higher than the effective concentration of Zn 2+ to hinder S. mutans and saliva-derived multi-species biofilm formation on material surfaces. Significance: The GIC containing BioUnion filler was shown to have the potential to inhibit biofilm formation in the oral cavity. In addition, recharging Zn 2+ and F − would further enhance the effect of the GIC containing BioUnion filler.