Expression profile of miRNAs in urine samples of bladder cancer patients. (3rd December 2018)

• Record Type: Journal Article
• Authors: Nekoohesh, Leila; Modarressi, Mohammad Hossein; Mowla, Seyed Javad; Sadroddiny, Esmaeil; Etemadian, Masoud; Afsharpad, Mandana; Zolfaghari, Faezeh; Barzegari, Manijeh; Saffari, Mojtaba; Oskooei, Vahid Kholghi; Motevaseli, Elahe; Ghafouri-Fard, Soudeh
• Is Part Of: Biomarkers in medicine. Volume 12:Number 12(2018)
• Page Start: 1311
• Page End: 1321
• Publication Date: 2018-12-03
• Subjects: biomarker -- bladder cancer -- expression -- microRNA -- miR-103 -- miR-10b -- miR-141 -- miR-34b -- painless hematuria -- recurrence; Biochemical markers -- Periodicals; 610.28
• DOI: 10.2217/bmm-2018-0190
• Languages: English
• ISSNs: 1752-0363

Abstract

Aim: miRNAs have been suggested as biomarkers for bladder cancer. We aimed to find a diagnostic panel of miRNAs based on differential expression of miRNAs in urine specimens of patient with bladder cancer compared with control group.Methods: miR-141, miR-10b, miR-34b and miR-103 were selected to assess their expression in urine samples of 66 bladder cancer patients and 53 matched controls using quantitative real time PCR.Results: miR-10b and miR-34b were upregulated in cases compared with controls. The combination of four miRNAs showed a sensitivity of 75% and specificity of 63.5% with a diagnostic power of 72%.Conclusion: Certain miRNAs can be used as biomarkers for early diagnosis of bladder cancer.