Platelet consumption and hyperreactivity coexist in experimental traumatic hemorrhagic model. (17th August 2020)

• Record Type: Journal Article
• Authors: Wannberg, Marcus; Miao, Xinyan; Li, Nailin; Wikman, Agneta; Wahlgren, Carl-Magnus
• Is Part Of: Platelets. Volume 31:Number 6(2020)
• Page Start: 777
• Page End: 783
• Publication Date: 2020-08-17
• Subjects: Animal model -- coagulopathy -- hemorrhage -- platelets -- shock -- trauma; Blood platelets -- Periodicals; Blood Platelets -- Periodicals; 615.39
• DOI: 10.1080/09537104.2019.1678120
• Languages: English
• ISSNs: 0953-7104

Abstract

Abstract: Introduction: Platelets are critical for hemostasis, and a low platelet count predicts mortality in trauma. The role of platelet dysfunction in severe traumatic hemorrhage and coagulopathy needs to be further defined. The aim of this study was to evaluate the platelet function in a new model of experimental traumatic hemorrhage. Material and methods: New Zealand white rabbits (n = 10) were subjected to tracheostomy and trauma laparotomy, and then bilateral femur fractures with 40% hemorrhage of their estimated blood volume. Arterial blood gases, standard coagulation tests, mean platelet volume, platelet aggregation using impedance aggregometry with agonist collagen, arachidonic acid (ASPI), and adenosine diphosphate (ADP), rotational thromboelastometry, and fibrinogen binding of platelets were analyzed using flow cytometry. Results: After traumatic hemorrhage, there was a significant physiological response with a rise in lactate ( P < .001) and a decrease in base excess ( P < .001) and temperature ( P < .001). Platelet count decreased from a mean of 244x10 9 /L to 94 x10 9 /L ( P = .004) and the mean platelet volume increased from 5.1fL to 6.1fL ( P = .002). Impedance aggregometry with the agonist collagen, ASPI, and ADP was all significantly decreased after hemorrhage ( P = .007). However, there was an increased fibrinogen binding of ADP-activated platelets after traumatic hemorrhage analyzed by flow cytometry ( P < .05). Conclusions: This traumatic hemorrhage model presents two parallel pathophysiological responses of platelets; platelet consumption as evidenced by a significant decrease in platelet count and aggregation, and platelet hyperreactivity as shown by a higher mean platelet volume and enhanced platelet fibrinogen binding. Further studies are needed to characterize these different aspects of platelet function in severe traumatic hemorrhage.