Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes. (2nd October 2020)

• Record Type: Journal Article
• Authors: Sajid, Mohammad; Biswas, Krishna; Singh, Harpreet; Negi, Sapna
• Is Part Of: Autoimmunity. Volume 53:Number 7(2020)
• Page Start: 385
• Page End: 393
• Publication Date: 2020-10-02
• Subjects: Gut microbes -- gut leakage -- serum cross-reactivity -- autoimmunity -- late-onset diabetes; Autoimmunity -- Periodicals; Autoimmune diseases -- Periodicals; 571.973
• DOI: 10.1080/08916934.2020.1818232
• Languages: English
• ISSNs: 0891-6934

Abstract

Abstract: The depletion of gut mucosal barrier enables exposure of gut microbes/gut microbial products to the host mucosal immunity which may increase the risk of metabolic/inflammatory disorders. These immune responses can lead to the development of mild autoimmunity to metabolic peptides coming from gut bacteria and may result in metabolic diseases like late-onset diabetes (LOD). In the present study, we identified host sera cross-reactivity with gut bacterial peptides similar to host proteins. The interaction between diabetic sera and gut peptides was detected by enzyme-linked immunosorbent assay (ELISA) and results were confirmed using surface plasmon resonance (SPR). The ELISA assay showed a higher level of serum cross-reactivity in LOD patients as compared to non-diabetic controls against three peptides (P-5, P-9, and P-13). SPR analysis confirmed binding-affinity against P-5 and P-13. Also, a significant correlation was observed between inflammatory markers and P-5. This study demonstrates that gut health is important not only for intestinal diseases but also for several late-onset diseases, like, diabetes.