Function analysis and stress-mediated cis-element identification in the promoter region of VqMYB15. (2nd July 2020)

• Record Type: Journal Article
• Authors: Li, Ruixiang; Zhu, Fanding; Duan, Dong
• Is Part Of: Plant signaling & behavior. Volume 15:Number 7(2020)
• Publication Date: 2020-07-02
• Subjects: Grapevine (Vitis quinquangularis) -- MYB15 promoter -- functional analysis -- GUS gene; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2020.1773664
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: The transcription factor MYB15 plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of cis -elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative cis -regulatory elements present upstream of MYB15 in Vitis quinquangularis Shanyang ( pVqMYB15 _SY) and subsequently characterized the function of these elements using reporter assays. Our results showed that TCA-elements 1 and 2, ABRE, MYC and 3-AF1 binding site 1 are key cis -regulatory elements in pVqMYB15 _SY and play important roles in plant bio/abiotic stress resistance.