Calcitonin-loaded octamaleimic acid–silsesquioxane nanoparticles in hydrogel scaffold support osteoinductivity in bone regeneration. (7th February 2021)

• Record Type: Journal Article
• Authors: Ahmadipour, Saeedeh; Varshosaz, Jaleh; Hashemibeni, Batool; Safaeian, Leila; Manshaei, Maziar; Sarmadi, Akram
• Is Part Of: Pharmaceutical development and technology. Volume 26:Number 2(2021)
• Page Start: 220
• Page End: 232
• Publication Date: 2021-02-07
• Subjects: Scaffold -- octamaleimic acid-silsesquioxane -- calcitonin -- bone tissue engineering -- hydrogel -- hydroxyapatite; Drug delivery systems -- Periodicals; Pharmaceutical technology -- Periodicals; Drugs -- Administration -- Research -- Periodicals; Drug Delivery Systems -- Periodicals; Pharmaceutical Preparations -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615
• DOI: 10.1080/10837450.2020.1858318
• Languages: English
• ISSNs: 1083-7450

Abstract

Abstract: Novel osteoinductive scaffolds fabricated using the benefits of tissue engineering techniques accompanied by utilizing drugs can accelerate bone regeneration. The purpose of this study was to load salmon calcitonin (sCT) in octamaleimic acid-silsesquioxane (OMA-POSS) nanoparticles and enrich the hydrogel scaffold based on hydroxyapatite, Gelrite® and platelet-rich plasma (PRP) for use in bone tissue engineering. The loading efficiency, release percentage, particle size and zeta potential of the nanoparticles were evaluated. The proliferation of seeded MG-63 osteoblast cells on the designed scaffold, its cytotoxicity and osteo-conductivity were studied by alkaline phosphatase measurement and Alizarin red staining. The expression of cellular osteogenic markers such as collagen 1 (COL1A1), osteocalcin (BGLAP) and osteopontin (SPP1) was examined using reverse transcription polymerase chain reaction. The results revealed that the particle size of the nanoparticles varied between 94.2 and 199.2 nm and their negative surface charge increased after drug conjugation. The osteoblast cell proliferation and calcium granule production in the optimum formulation were significantly higher in comparison with the control group ( p < 0.05). Osteogenic markers increased significantly after a specific number of days of cell culture compared to the control group ( p < 0.05). The results also showed the potential of the designed scaffold in bone tissue engineering.