Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers. (2nd October 2020)

• Record Type: Journal Article
• Authors: Busuttil-Crellin, Xavier; McCafferty, Conor; Van Den Helm, Suelyn; Yaw, Hui Ping; Monagle, Paul; Linden, Matthew; Ignjatovic, Vera
• Is Part Of: Platelets. Volume 31:Number 7(2020)
• Page Start: 845
• Page End: 852
• Publication Date: 2020-10-02
• Subjects: Flow Cytometry -- Paediatrics -- Panel Design and Optimization -- Platelet; Blood platelets -- Periodicals; Blood Platelets -- Periodicals; 615.39
• DOI: 10.1080/09537104.2019.1709630
• Languages: English
• ISSNs: 0953-7104

Abstract

Abstract: Flow cytometry is a valuable tool in determining the phenotype and function of platelets accurately. The emergence of platelet flow cytometry in recent years provides an attractive alternative to other platelet analytical techniques, with advantages such as requiring small volumes and being highly sensitive to minimal changes in receptor function and expression. Here we present a methodical approach encompassing the stages in the development and optimization of platelet flow cytometry panels based on our extensive experience in this area.