Simultaneous Determination of Uric Acid and Xanthine Using a Poly(Methylene Blue) and Electrochemically Reduced Graphene Oxide Composite Film Modified Electrode. (11th November 2014)

• Record Type: Journal Article
• Authors: Liu, Gen; Ma, Wei; Luo, Yan; Sun, Deng-ming; Shao, Shuang
• Other Names: Huang Chih-Ching Academic Editor.
• Is Part Of: Journal of analytical methods in chemistry. Volume 2014(2014)
• Publication Date: 2014-11-11
• Subjects: Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Technique -- Periodicals; 543.05
• DOI: 10.1155/2014/984314
• Languages: English
• ISSNs: 2090-8865

Abstract

Abstract : Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10 −8 ~4.00 × 10 −4 M and 1.00 × 10 −7 ~4.00 × 10 −4 M, respectively, by SWV. The detection limits for UA and Xa were 3 . 00 × 10 - 8 M and 5.00 × 10 - 8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.