Cytotoxicity of Carbon Nanotubes on J774 Macrophages Is a Purification-Dependent Effect. (27th June 2012)

• Record Type: Journal Article
• Authors: Montes-Fonseca, Silvia Lorena; Orrantia-Borunda, Erasmo; Duarte-Möller, Alberto; Luna-Velasco, Antonia; Román-Aguirre, Manuel; González Horta, Carmen; Sánchez-Ramírez, Blanca
• Other Names: Vasilev Krasimir Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2012(2012)
• Publication Date: 2012-06-27
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2012/715838
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : The cytotoxicity of the carbon nanotubes (CNTs) is an important factor for the manufacture of nanovaccines. The aim of this work was to evaluate the relationship of the purification method of CNTs in cellular toxicity using macrophages (MOs) from the J774 cell line. Viability test was performed with MTT assays at 24 h of exposure at concentrations of 0.06, 0.6, and 6 mg/L of unpurified (UP-CNTs) or purified (P-CNTs) CNTs by two different methods: (1) reflux with 3M HNO3 and (2) sonication in H2 SO4 /HNO3 . Characterization and COOH content of CNTs was performed using scanning electron microscopy, raman spectroscopy, and titration with NaHCO3 . P-CNTs1 had lengths >100 μ m and 2.76% COOH content, while P-CNTs2 had lengths >1 μ m and 7% COOH content. This last particle showed a lower toxic effect. The results suggest that the lenght and COOH content are important factors in the toxicity of the CNTs.