Fecal Colonization with Extended-Spectrum Beta-Lactamase and AmpC-Producing Escherichia coli. (31st May 2016)

• Record Type: Journal Article
• Authors: Al-Agamy, Mohamed H.; El Mahdy, Taghrid S.; Shibl, Atef M.
• Other Names: Mansour Wejdene Academic Editor.
• Is Part Of: BioMed research international. Volume 2016(2016)
• Publication Date: 2016-05-31
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2016/3704150
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Background . Extended-spectrum β -lactamases (ES β Ls) and AmpC β -lactamases cause β -lactam resistance in Escherichia coli . Fecal colonization by ES β L- and/or AmpC-positive E. coli is a source of nosocomial infections. Methods . In order to investigate inpatient fecal colonization by ES β Ls and AmpC, antibiotic sensitivity tests were conducted and minimum inhibitory concentrations (MICs) were determined using the disk diffusion method and E -test, respectively. Characterization of ES β L and AmpC was performed using E -test strips, and a set of PCRs and DNA sequence analyses were used to characterize the ES β L and AmpC genes. Results . The whole collection of E. coli isolates (n = 50 ) was sensitive to imipenem, tigecycline, colistin, and fosfomycin, while 26% of the isolates showed reduced susceptibility to ceftazidime (MIC ≥ 4 μ g/mL). ES β L was phenotypically identified in 26% (13/50) of cases, while AmpC activity was detected in two ES β L-producing E. coli isolates. All ES β L-producing E. coli were positive for the CTX-M gene, eleven isolates carried b l a CTX-M-15, and two isolates carried b l a CTX-M-14 gene. Two CTX-M-positive E. coli isolates carried b l a CMY-2 . Conclusions . The alimentary tract is a significant reservoir for ES β L- and/or AmpC-producing E. coli, which may lead to nosocomial infection.