Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization. (21st December 2014)

• Record Type: Journal Article
• Authors: Cárdaba, Blanca; Calzada, David; Baos, Selene; Aguerri, Miriam; Quiralte, Joaquín; Lahoz, Carlos
• Other Names: Blaser Kurt Academic Editor.
• Is Part Of: Journal of immunology research. Volume 2014(2014)
• Publication Date: 2014-12-21
• Subjects: Immunology -- Periodicals; Immunology -- Research -- Periodicals; 616.07905
• DOI: 10.1155/2014/276345
• Languages: English
• ISSNs: 2314-8861

Abstract

Abstract : Sensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims of TNFA ( G-308A, C-857T, and C-1031T ), IL10 ( C-571A and A-1117G ), and TGFB ( C-509-T ) and these sensitizations. These polymorphisms were genotyped by allelic discrimination, in olive pollen-allergic patients (phenotyped for specific Ole e 2 and 10 sensitizations) and healthy controls. Levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. The results showed that heterozygous TGFB C-509T genotype, besides having the lowest sera TGF- levels, was significantly increased in olive pollen-allergic patients compared with controls. According specific sensitizations, CC genotype of IL10 C - 571A could be a protective factor for Ole e 2 sensitization and mainly for asthmatic Ole e 2 sensitized patients compared with asthmatic non-Ole e 2 sensitized patients (OR: 0.26, P = 0.008 ). In contrast, heterozygous CA genotype was increased in Ole e 2 asthmatic subjects compared to asthmatic non-Ole e 2 sensitized patients. Lastly, heterozygous TNFA G-308A genotype was associated with Ole e 10 sensitization (OR: 2.5, P = 0.04 ). In conclusion, these results suggest a role of TGF- β 1 in olive-pollen sensitization and TNF- α and IL-10 genotypes in the asthma induced by specific olive-pollen allergens.