Magnetic Solid-Phase Extraction Using Fe3O4@SiO2 Magnetic Nanoparticles Followed by UV-Vis Spectrometry for Determination of Paraquat in Plasma and Urine Samples. (17th July 2017)

• Record Type: Journal Article
• Authors: Sha, Ou; Wang, Yu; Yin, Xin; Chen, Xiaobing; Chen, Li; Wang, Shujun
• Other Names: Pyrzynska Krystyna Academic Editor.
• Is Part Of: Journal of analytical methods in chemistry. Volume 2017(2017)
• Publication Date: 2017-07-17
• Subjects: Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Technique -- Periodicals; 543.05
• DOI: 10.1155/2017/8704639
• Languages: English
• ISSNs: 2090-8865

Abstract

Abstract : A rapid and simple method was optimized and validated for the separation and quantification of paraquat, a frequently used herbicide and a leading cause of fatal poisoning worldwide, at trace levels with UV-Vis spectrophotometry in plasma and urine samples by direct magnetic solid-phase extraction. Fe3 O4 @SiO2 nanoparticles (NPs) were used as the magnetic solid-phase extraction agents and the paraquat absorbed on NPs was eluted using NaOH and ascorbic acid. Upon optimization, paraquat could be extracted and concentrated from various samples by 35-fold. The linear range, limit of detection (LOD), correlation coefficient (R ), and relative standard deviation (RSD) could reach 15.0–400.0 μ g/L, 12.2 μ g/L, 0.9987, and 0.65% (n = 5, c = 40.0 μ g/L), respectively. The Fe3 O4 @SiO2 NPs could be reused up to five times. The method was successfully applied to the determination of paraquat in urine and plasma at different hemoperfusion numbers in a local hospital for the patient of paraquat poisoning. The experiment result could not only enable immediate medical intervention but also benefit patients' survival.