The Effect of Advanced Motherhood on Newborn Offspring's Hippocampal Neural Stem Cell Proliferation. (5th September 2016)

• Record Type: Journal Article
• Authors: Li, Bo; Duan, Ping; Han, Xuefei; Yan, Wenhai; Xing, Ying
• Other Names: von Bohlen und Halbach Oliver Academic Editor.
• Is Part Of: BioMed research international. Volume 2016(2016)
• Publication Date: 2016-09-05
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2016/6171352
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Objective . To investigate the effect of advanced motherhood on rat hippocampal neural stem cell proliferation. Methods . Female parents were subdivided into control and old mother group by age, and neural stem cells were cultured from hippocampal tissues for 24 h newborn offspring. The diameter and numbers of neurospheres were examined by microscopy, and differences in proliferation were examined by EdU immunofluorescence, CCK-8 assay, and cell cycle analysis. Results . The number of neurospheres in the old mother group after culture was lower than the control group. Additionally, neurospheres' diameter was smaller than that of the control group (P < 0.05 ). The EdU positive rate of the old mother group was lower than that of the control group (P < 0.05 ). CCK-8 assay results showed that the absorbance values for the old mother group were lower than that of the control group at 48 h and 72 h (P < 0.05 ). The proportions of cells in the S and G2/M phases of the cell cycle for the older mother group were less than that found for the control group (P < 0.05 ). Conclusion . The proliferation rates of hippocampal NSCs seen in the older mother group were lower than that seen in the control group.