New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa. (1st February 2012)

• Record Type: Journal Article
• Authors: Gomes-Santos, Ana Cristina; Moreira, Thais Garcias; Castro-Junior, Archimedes Barbosa; Horta, Bernardo Coelho; Lemos, Luisa; Cruz, Deborah Nogueira; Guimarães, Mauro Andrade Freitas; Cara, Denise Carmona; McCafferty, Donna-Marie; Faria, Ana Maria Caetano
• Other Names: Tsuji Noriko Academic Editor.
• Is Part Of: Clinical & developmental immunology. Volume 2012(2012)
• Publication Date: 2012-02-01
• Subjects: Developmental immunology -- Periodicals; Clinical immunology -- Periodicals; Immune System -- immunology -- Periodicals; Immune System -- growth & development -- Periodicals; Immune System Diseases -- immunology -- Periodicals; 571.9638
• DOI: 10.1155/2012/560817
• Languages: English
• ISSNs: 1740-2522

Abstract

Abstract : IL-10 is a regulatory cytokine that plays a major role in the homeostasis of the gut and this is illustrated by the fact that IL-10 −/− mice develop spontaneous colitis. In this study, IL-10 −/− mice were analyzed for immunological changes during colitis development. We found a reduced frequency of regulatory T cells CD 4 + CD 25 + Foxp 3 + and higher frequency of activated T cells in the colon that precedes the macroscopic signs of the disease. Production of IL-17 and IFN-γ was higher in the colon. Colitis progression culminates with the reduction of CD 4 + LAP + regulatory T cells in the intestine. Frequency of B1 cells and the secretory IgA production were both elevated. Despite these alterations, 16-week-old IL-10 −/− mice could be rendered tolerant by a continuous feeding protocol. Our study provides detailed analysis of changes that precede colitis and it also suggests that oral tolerance could be used to design novel alternative therapies for the disease.