Bismuth-based nanoparticles impair adipogenic differentiation of human adipose-derived mesenchymal stem cells. (December 2021)

• Record Type: Journal Article
• Authors: Ribeiro, Annanda Lyra; Bassai, Letícia Werzel; Robert, Anny Waloski; Machado, Thiago Neves; Bezerra, Arandi Ginane; Horinouchi, Cintia Delai da Silva; Aguiar, Alessandra Melo de
• Is Part Of: Toxicology in vitro. Volume 77(2022)
• Publication Date: 2021-12
• Subjects: Nanoparticles -- Bismuth -- Stem cells -- Cell differentiation; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2021.105248
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Bismuth-based nanoparticles (BiNPs) have attracted attention for their potential biomedical applications. However, there is a lack of information concerning their interaction with biological systems. In this study, it was investigated the effect of physically synthesized BiNPs to human adipose-derived stem cells (ADSCs). We first evaluated the influence of BiNPs on cell viability, cell morphology, mitochondrial function and cell proliferation. Further, the impact of BiNPs on adipogenic differentiation was also explored. Cytotoxicity assays have demonstrated that BiNPs did not reduce relative cell viability of ADSC except at the highest tested concentration (345 μg/ml). Analysis of cell morphology performed by transmission electron microscopy confirmed that BiNPs induced cell damage only at a high concentration (302.24 μg/ml), equivalent to IC50 concentration. Moreover, BiNPs exposure increased the expression of the cell proliferation marker Ki-67 and the incorporation of the thymidine analogue EdU into cell DNA, suggesting that these nanoparticles could be stimulating ADSC proliferation. BiNPs also increased the mitochondrial membrane potential. Furthermore, BiNPs reduced ADSC adipogenic differentiation as measured by lipid droplet accumulation and mRNA expression levels of the specific adipogenesis biomarkers PPARγ, C/EPBɑ and FABP4. Thus, BiNPs affect the nonspecific (viability, proliferation and mitochondrial activity) and specific (adipogenesis) cellular mechanisms of ADSCs. Graphical abstract: Unlabelled Image Highlights: BiNP batches produced by LASiS have similar physicochemical characteristics. BiNPs induce ADSC proliferation and mitochondrial activity. BiNPs inhibit ADSC adipogenesis in a dose-dependent manner.