Conditional knockout of Cdc20 attenuates osteogenesis in craniofacial bones. (August 2022)

• Record Type: Journal Article
• Authors: Cheng, Yawen; Du, Yangge; Zhang, Xiao; Zhang, Ping; Liu, Yunsong
• Is Part Of: Tissue & cell. Volume 77(2022)
• Publication Date: 2022-08
• Subjects: ALP Alkaline phosphatase -- APC/C Anaphase-promoting complex/cyclosome -- BV/TV Bone volume/total volume -- BMD Bone mineral density -- CCK8 Cell Counting Kit-8 -- CDC20 Cell division cycle protein 20 -- CRISPR Clustered regularly interspaced short palindromic repeats -- EGE Extreme genome editing -- H&E Hematoxylin and eosin -- Micro-CT Micro-computed tomography -- MSCs Mesenchymal stem cells -- OD Optical density -- OM Osteogenic medium -- Osx Osterix -- PBS Phosphate-buffered saline -- PM Proliferation medium -- qRT-PCR Quantitative real-time polymerase chain reaction -- ROI Region of interest -- sgRNAs Single guide RNAs -- Sp7 Sp7 transcription factor 7 -- Tb.N trabecular number -- Tb. Sp Trabecular spacing; CDC20 -- Cranial bone -- Mandibular bone -- Mesenchymal stem cells -- Osteogenesis; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2022.101829
• Languages: English
• ISSNs: 0040-8166

Abstract

Abstract: Craniofacial bone defects cause significant problems to patients with harmful consequences. Mesenchymal stem cells (MSCs) can self-renew and exhibit multilineage differentiation, which could be applied to bone regeneration. However, craniofacial bone tissue MSCs have unique properties, differing in their characteristics to MSCs derived from long bones. CDC20 promotes osteogenic differentiation in long bones; however, its role in craniofacial bone tissues remains unknown. In this study, we found that Cdc20 conditional knockout in mice triggered distinctive cranial and mandibular bone loss. Moreover, the osteogenic differentiation potential of cranial suture-derived MSCs and mandibular bone marrow-derived MSCs was impaired in Cdc20 conditional knockout mice. The conditional knockout of Cdc20 impaired osteogenesis in craniofacial bones. Our findings provide new insights into craniofacial bone regeneration and the treatments of craniofacial bone-related diseases. Graphical Abstract: ga1 Highlights: Sp7-Cre;Cdc20 f/f mice were used to study the role of CDC20 on craniofacial bones. Conditional knockout of Cdc20 decreased craniofacial bone formation. Lack of Cdc20 inhibited osteogenic differentiation in craniofacial MSCs.