20-Hydroxyecdysone-responsive microRNAs of insects. Issue 10 (2nd October 2020)

• Record Type: Journal Article
• Authors: Jin, Xiaoli; Wu, Xiaoyan; Zhou, Lanting; He, Ting; Yin, Quan; Liu, Shiping
• Is Part Of: RNA biology. Volume 17:Issue 10(2020)
• Page Start: 1454
• Page End: 1471
• Publication Date: 2020-10-02
• Subjects: 20-hydroxyecdysone -- ecdysone-signalling pathway -- microRNA -- insect -- Bombyx mori -- Drosophila melanogaster; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2020.1775395
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: 20-Hydroxyecdysone (20-HE) plays essential roles in coordinating developmental transitions of insects through responsive protein-coding genes and microRNAs (miRNAs). The involvement of single miRNAs in the ecdysone-signalling pathways has been extensively explored, but the interplay between ecdysone and the majority of miRNAs still remains largely unknown. Here, by small RNA sequencing, we systematically investigated the genome-wide responses of miRNAs to 20-HE in the embryogenic cell lines of Bombyx mori and Drosophila melanogaster . Over 60 and 70 20-HE-responsive miRNAs were identified in the BmE cell line and S2 cell line, respectively. The response of miRNAs to ecdysone exhibited a time-dependent pattern, and the response intensity increased with extending exposure to 20-HE. The relationship between ecdysone and the miRNAs was further explored through knockdown of ecdysone-signalling pathway genes. Specifically, ecdysone regulated the cluster miR-275 and miR-305 through the coordination of BmEcR-B and downstream BmE75B, and the interaction between BmEcR and miR-275 cluster was strengthened by the feedback regulation of BmE75B . Ecdysone induced miR-275-3p and miR-305-5p through the ecdysone response effectors (EcREs) at the upstream of the pre-miR-275 cluster. Overall, the results might help us further understand the relationship between ecdysone signalling pathways and small RNAs in the development and metamorphosis of insects.