Utilization of industrial citrus pectin side streams for enzymatic production of human milk oligosaccharides. (September 2022)

• Record Type: Journal Article
• Authors: Biel-Nielsen, Tessa L.; Li, Kai; Sørensen, Susanne O.; Sejberg, Jimmy J.P.; Meyer, Anne S.; Holck, Jesper
• Is Part Of: Carbohydrate research. Volume 519(2022)
• Publication Date: 2022-09
• Subjects: Industrial depectinized citrus side stream -- Fucosylated xyloglucan -- Enzymatic extraction -- Human milk oligosaccharides; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2022.108627
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: This study reports the enzymatic upgrading of fucosylated xyloglucan from depectinized citrus residues into 2′-fucosyllactose, a fucosylated human milk oligosaccharide. Alkaline and enzymatic xyloglucan extractions were compared. Of the original fucose present in the depectinized residues of lemon and orange, 35–36% and 48–51% were extracted as fucosylated xyloglucan by enzyme- or alkaline treatment, respectively. Furthermore, the enzymatically extracted xyloglucan structures had a narrower molecular weight distribution around 1 kDa, contrary to a more polydisperse distribution of the alkaline extracted xyloglucans, ranging from 1 to 500 kDa. The applicability of the fucosylated-xyloglucan extracts in transfucosylation reactions, was determined by use of a selected fungal fucosidase, resulting in yields of 10.2–11.4% enzymatic extracts, and 6.5–7.4% for alkaline extracts (orange and lemon respectively). The results demonstrate that depectinized citrus side streams are a useful source of fucosylated xyloglucan, preferably extracted by an enzyme catalyzed approach. Graphical abstract: Image 1 Highlights: Depectinized citrus side streams are useful sources of fucosylated xyloglucan. Lemon and orange side streams are equally good sources of fucosylated xyloglucan. Treatment with Cellic CTec3 successfully extracted fucosylated xyloglucans. Enzymatically extracted xyloglucans are superior for transfucosylation reactions.