Mimicking the Brain Extracellular Matrix in Vitro: A Review of Current Methodologies and Challenges. Issue 12 (10th July 2019)

• Record Type: Journal Article
• Authors: Rauti, Rossana; Renous, Noa; Maoz, Ben M.
• Is Part Of: Israel journal of chemistry. Volume 60:Issue 12(2020)
• Page Start: 1141
• Page End: 1151
• Publication Date: 2019-07-10
• Subjects: brain extracellular matrix -- microenvironment -- In vitro neuronal cultures -- perineuronal nets -- Biocompatible materials; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/ijch.201900052
• Languages: English
• ISSNs: 0021-2148

Abstract

Abstract: The brain's extracellular matrix (ECM), a 3‐dimensional macromolecular network that supports cell growth and viability, has crucial roles in homeostasis and disease. Accurate recapitulation of the chemical, structural, and mechanical properties of the ECM in brain cell cultures is essential for the development of translatable in vitro models; however, thus far, this task has proven highly challenging. This review provides an in‐depth discussion of this challenge, including an overview of the properties of the ECM that in vitro models should endeavour to capture, a survey of ECM analogues that are currently used for this purpose, and a discussion of the main hindrances to developing more effective ECM‐like coating materials. Abstract :