Ligand fishing based on tubular microchannel modified with monoamine oxidase B for screening of the enzyme's inhibitors from Crocus sativus and Edgeworthia gardneri. Issue 13 (3rd May 2022)

• Record Type: Journal Article
• Authors: Qi, Xu‐Wei; Liu, Yi‐Ming; Hu, Yi‐Kao; Yuan, Hao; Ayeni, Emmanuel Ayodeji; Liao, Xun
• Is Part Of: Journal of separation science. Volume 45:Issue 13(2022)
• Page Start: 2394
• Page End: 2405
• Publication Date: 2022-05-03
• Subjects: bioactive compounds -- ligand fishing -- modified microchannel -- monoamine oxidase B -- screening; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.202200057
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : A novel strategy of performing ligand fishing with enzyme‐modified open tubular microchannel was proposed for screening bioactive components present in medicinal plants. Monoamine oxidase B was immobilized onto the surface of the microchannel for the first time to specifically extract its ligands when the plant's extracts solution flows through the channel. The thermal and the storage stability of immobilized monoamine oxidase B were significantly enhanced after immobilization. Crocin I and Ⅱ were extracted from Crocus sativus, and tiliroside was extracted from Edgeworthia gardneri . All the three compounds were inhibitors of the enzyme with the half‐maximal inhibitory concentration values of 26.70 ± 0.91, 19.88 ± 2.78, and 15.65 ± 0.85 μM, respectively. The enzyme inhibition kinetics and molecular docking were investigated. This is the first report on the inhibitory effects of tiliroside and crocin Ⅱ. The novel ligand fishing method proposed in this work possesses advantages of rapidness, high efficiency, and tiny sample consumption compared to routine ligand fishing, with promising potential for screening active natural products in complex mixtures.