Bacteriostatic effects of phage F23s1 and its endolysin on Vibrio parahaemolyticus. (3rd June 2022)

• Record Type: Journal Article
• Authors: Xia, Hai; Yang, Houji; Yan, Na; Hou, Wenfu; Wang, Huajuan; Wang, Xiaohong; Wang, Hongxun; Zhou, Min
• Is Part Of: Journal of basic microbiology. Volume 62:issue 8(2022)
• Page Start: 963
• Page End: 974
• Publication Date: 2022-06-03
• Subjects: endolysin -- phage -- shrimp -- Vibrio parahaemolyticus; Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.202200056
• Languages: English
• ISSNs: 0233-111X

Abstract

Abstract: Vibrio parahaemolyticus is a common foodborne pathogenic bacterium and drug‐resistant strains are now widespread. Phages led by drug‐resistant V. parahaemolyticus strains are promising means to decrease the pressure on public health. We isolated a V. parahaemolyticus ‐specific bacteriophage F23s1 that was active at wide ranges of temperature (30–60°C) and pH (4–10). Phage F23s1 exhibited a specific host range; in that, only 13 of the 23 V. parahaemolyticus strains were lysed. F23s1 effectively inhibited the growth of V. parahaemolyticus strain F23 in shrimp at 25°C within 12 h at a multiplicity of infection of 1000. We sequenced the genome of phage F23s1 which comprised a 76, 648‐bp DNA with 105 open reading frames (ORFs) and identified an endolysin gene ORF52 that was then cloned and successfully expressed in Escherichia coli . The recombinant ORF52 protein significantly decreased OD600 nm of V. parahaemolyticus F23 from 0.978 to 0.249 when used at 20 µmol/L within 60 min. The endolysin also showed lytic activity against a panel of 23 drug‐resistant V. parahaemolyticus and 12 Salmonella strains with a higher lytic ability for V. parahaemolyticus . The phage F23s1 and its endolysin will be useful for preventing and controlling V. parahaemolyticus in food safety.