LncPVT1 regulates osteogenic differentiation of human periodontal ligament cells via miR‐10a‐5p/brain‐derived neurotrophic factor. Issue 7 (19th January 2022)

• Record Type: Journal Article
• Authors: Li, Xiaobei; Huang, Yiping; Han, Yineng; Yang, Qiaolin; Zheng, Yunfei; Li, Weiran
• Is Part Of: Journal of periodontology. Volume 93:Issue 7(2022)
• Page Start: 1093
• Page End: 1106
• Publication Date: 2022-01-19
• Subjects: brain‐derived neurotrophic factor -- RNA, long noncoding -- microRNAs -- osteogenesis -- periodontal ligament -- stem cells; Periodontics -- Periodicals; 617.632
• DOI: 10.1002/JPER.21-0429
• Languages: English
• ISSNs: 0022-3492

Abstract

Abstract: Background: Identifying the factors affecting osteoblast differentiation of periodontal ligament cells (PDLCs) can help enhance the regeneration of periodontal tissue. LncRNA plasmacytoma variant translocation 1 (lncPVT1) is an important regulatory factor involved in many biological processes, but its role in osteogenesis remains unclear. Methods: Expressions of osteogenic markers were detected by quantitative reverse transcription polymerase chain reaction and Western blot analysis. Alkaline phosphatase staining was conducted for early osteoblast differentiation and alizarin red S staining was used for mineral deposition. RNA sequencing was used to identify the miRNAs regulated by lncPVT1 during osteogenesis. Cell transfection was used to overexpress or knockdown lncPVT1 and miR‐10a‐5p. Dual luciferase reporter assays were conducted to analyze the binding of miR‐10a‐5p to brain‐derived neurotrophic factor (BDNF). Results: LncPVT1 was significantly increased during osteogenic induction of PDLCs. Overexpression of lncPVT1 promoted osteogenesis, whereas lncPVT1 knockdown inhibited this process. RNA sequencing showed that miR‐10a‐5p expression was significantly increased after lncPVT1 knockdown. RNA immunoprecipitation assay further demonstrated the binding potential of lncPVT1 and miR‐10a‐5p. MiR‐10a‐5p inhibited the osteogenesis of PDLCs, and partially reversed the stimulatory effects of lncPVT1. Subsequently, we identified a predicted binding site for miR‐10a‐5p on BDNF and confirmed it using dual luciferase reporter assays. Moreover, lncPVT1 upregulated the expression of BDNF, whereas miR‐10a‐5p downregulated BDNF expression. BDNF promoted osteogenesis and partially rescued the si‐lncPVT1‐mediated inhibition of PDLCs osteogenic differentiation. Conclusions: LncPVT1 positively regulated the osteogenic differentiation of PDLCs via miR‐10a‐5p and BDNF. Our results provide a promising target for enhancing the osteogenic potential of PDLCs.