Synthesis of 2‐Chloro‐3‐amino indenone derivatives and their evaluation as inhibitors of DNA dealkylation repair. (7th April 2021)

• Record Type: Journal Article
• Authors: Nigam, Richa; Raveendra Babu, Kaki; Ghosh, Topi; Kumari, Bhavini; Das, Prolay; Anindya, Roy; Ahmed Khan, Faiz
• Is Part Of: Chemical biology & drug design. Volume 97:Number 6(2021)
• Page Start: 1170
• Page End: 1184
• Publication Date: 2021-04-07
• Subjects: AlkB -- dioxygenase -- DNA alkylation -- DNA repair -- indenone; Drugs -- Design -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; 615.19005
• DOI: 10.1111/cbdd.13839
• Languages: English
• ISSNs: 1747-0277

Abstract

Abstract: DNA alkylation damage, emanating from the exposure to environmental alkylating agents or produced by certain endogenous metabolic processes, affects cell viability and genomic stability. Fe(II)/2‐oxoglutarate‐dependent dioxygenase enzymes, such as Escherichia coli AlkB, are involved in protecting DNA from alkylation damage. Inspired by the natural product indenone derivatives reported to inhibit this class of enzymes, and a set of 2‐chloro‐3‐amino indenone derivatives was synthesized and screened for their inhibitory properties against AlkB. The synthesis of 2‐chloro‐3‐amino indenone derivatives was achieved from 2, 3‐dichloro indenones through addition–elimination method using alkyl/aryl amines under catalyst‐free conditions. Using an in vitro reconstituted DNA repair assay, we have identified a 2‐chloro‐3‐amino indenone compound 3o to be an inhibitor of AlkB. We have determined the binding affinity, mode of interaction, and kinetic parameters of inhibition of 3o and tested its ability to sensitize cells to methyl methanesulfonate that mainly produce DNA alkylation damage. This study established the potential of indenone‐derived compounds as inhibitors of Fe(II)/2‐oxoglutarate‐dependent dioxygenase AlkB. Abstract : Natural product indenone‐derived compound inhibits DNA repair enzyme AlkB.