A CRISPR/Cas9-mediated gene knockout system in Aspergillus luchuensis mut. kawachii. Issue 10 (2nd October 2020)

• Record Type: Journal Article
• Authors: Kadooka, Chihiro; Yamaguchi, Masaaki; Okutsu, Kayu; Yoshizaki, Yumiko; Takamine, Kazunori; Katayama, Takuya; Maruyama, Jun-Ichi; Tamaki, Hisanori; Futagami, Taiki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 84:Issue 10(2020)
• Page Start: 2179
• Page End: 2183
• Publication Date: 2020-10-02
• Subjects: Aspergillus luchuensis mut. kawachii -- shochu -- CRISPR/Cas9 -- genome editing -- AMA1; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2020.1792761
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: We developed an approach to genome editing of the white koji fungus, Aspergillus luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Co-transformation of AMA1 -based Cas9 and gRNA expression plasmids achieved efficient gene knockout in A. kawachii . The plasmids were easily lost when selective pressure was removed, allowing for successive rounds of genome editing.