Communication—Direct Detection of Methyl Salicylate Using Tri-Enzyme Based Electrochemical Sensor. Issue 9 (1st January 2018)

• Record Type: Journal Article
• Authors: Fang, Yi; Zhou, Yan; Ramasamy, Ramaraja P.
• Is Part Of: Journal of the Electrochemical Society. Volume 165:Issue 9(2018)
• Page Start: B358
• Page End: B360
• Publication Date: 2018-01-01
• Subjects: biosensor -- methyl salicylate -- volatile organic compound; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/2.0541809jes
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : A new method for direct detection of methyl salicylate, a key volatile chemical marker for biotic stresses in plants is described. The approach involves a 4-reaction cascade that converts methyl salicylate into 1, 2-benzoquinone using esterase, salicylate hydroxylase and tyrosinase, where 1, 2-benzoquinone is electrochemically reduced to catechol resulting in an amperometric signal. The approach vastly simplifies the procedure for amperometric detection of methyl salicylate described earlier by eliminating the need for cumbersome pretreatment procedures for the analyte. Initial data suggested that the tri-enzyme based detection is promising over the previously reported bi-enzyme based methods.