Antiviral effects and possible mechanisms of action of constituents from Brazilian propolis and related compounds. Issue 4 (7th August 2020)

• Record Type: Journal Article
• Authors: Kwon, Min Jung; Shin, He Min; Perumalsamy, Haribalan; Wang, Xue; Ahn, Young-Joon
• Is Part Of: Journal of apicultural research. Volume 59:Issue 4(2020)
• Page Start: 413
• Page End: 425
• Publication Date: 2020-08-07
• Subjects: propolis -- human rhinovirus -- p-coumaric acid -- kaempferol -- mechanism of action -- natural antiviral agent; Bee culture -- Periodicals; Bee culture; Periodicals; 638.105
• DOI: 10.1080/00218839.2019.1695715
• Languages: English
• ISSNs: 0021-8839

Abstract

Abstract : Propolis is a well-known medicinal bee hive product amongst others due to its antiviral properties. Human rhinoviruses (HRVs) are recognized to be responsible for more than 50% of common colds worldwide. This study was performed to determine if kaempferol (KF) and p -coumaric acid ( p -CA) extracted from Brazilian propolis and another eight previously known compounds of propolis had antiviral activity toward human rhinovirus (HRV)-2, HRV-3, and HRV-4 in HeLa cells and to determine their mechanism of action using sulforhodamine B assay and real-time reverse transcription polymerase chain reaction. Results were compared with those of a positive control ribavirin. Based on IC50 values, KF, quercetin, chrysin, and luteolin were more pronounced in antiviral activity toward HRV-2 (7.3–37.4 μM), HRV-3 (11.9–20.4 μM), and HRV-4 (12.9–24.4 μM) than a drug reference ribavirin (270.1–323.9 μM). The 35 μM KF and 610 μM p -CA did not interact with the HRV-3 particles. KF and p -CA inhibited HRV-3 infection when added during the early stages (0–4 h) after virus inoculation. These constituents remarkably reduced the RNA replication levels of HRV-3 in the HeLa cell cultures. These findings suggest that KF and p -CA may block or reduce the entrance of the viruses into the cells to protect the cells from the virus destruction and abate virus replication, which may play a crucial role in interfering with expressions of intercellular adhesion molecule-1 and interleukin-6. Further studies about the possible applications of propolis-derived materials as potential products to prevent or treat HRV infection are warranted.