Electrochemical Sensor for the Simultaneous Detection of Guanine and Adenine Based on a PPyox/MWNTs-MoS2 Modified Electrode. Issue 6 (1st January 2019)

• Record Type: Journal Article
• Authors: Geng, Xintong; Bao, Jing; Huang, Ting; Wang, Xiaojing; Hou, Changjun; Hou, Jingzhou; Samalo, Mickey; Yang, Mei; Huo, Danqun
• Is Part Of: Journal of the Electrochemical Society. Volume 166:Issue 6(2019)
• Page Start: B498
• Page End: B504
• Publication Date: 2019-01-01
• Subjects: Sensors -- Adenine -- Biosensor -- Guanine; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/2.1221906jes
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : Guanine (G) and adenine (A) are two important components of nucleic acid and some small molecules in an organism. In this paper, we developed a new and convenient biosensor for the simultaneous determination of G and A based on overoxidized polypyrrole/multi- walled carbon nanotube and molybdenum disulfide modified glassy carbon electrode (PPyox/ MWNTs-MoS2 /GCE). The composite material exhibited remarkable electro-catalytic properties toward the oxidation of G and A due to its large surface area and fast electron transfer. The oxidation peak positions of G and A were at 0.73 V and 1.03 V, respectively, which allowed simultaneous detection of the two substances in coexistence solution. The designed biosensor exhibited linear responses to G and A both in the ranges 5–30 μM and 30–120 μM, with detection limits (S/N = 3) of 1.6 μM (G) and 1.7 μM (A), respectively. Moreover, interferences from some coexisting electro-active species including inorganic ions, glucose, glycine and citric acid were nearly negligible. Further, the sensing strategy was successfully used for the detection of G and A in real samples with satisfactory results, indicating a potential application for the simultaneous electrochemical detection of G and A.