Characterization of the complete chloroplast genome of Bupleurum hamiltonii N. P. Balakr. (Apiaceae) and its phylogenetic implications. Issue 2 (1st February 2021)

• Record Type: Journal Article
• Authors: Zhang, Gaixia; Wang, Hui; Yin, Xin; Kong, Weijun; Wang, Qiuling; Chen, Yi; Wei, Jianhe; Liu, Jinxin; Guo, Xinwei
• Is Part Of: Mitochondrial DNA. Volume 6:Issue 2(2021)
• Page Start: 447
• Page End: 449
• Publication Date: 2021-02-01
• Subjects: Bupleurum hamiltonii -- chloroplast genome -- phylogenetic -- identification; Mitochondrial DNA -- Periodicals; 572.869
• DOI: 10.1080/23802359.2020.1870902
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: Bupleurum hamiltonii has a thin, wood, grayish-yellow root and is reputed to possess medicinal value. This study employs the de novo method using high-throughput sequencing data to assemble the complete chloroplast genome for this species. The total B. hamiltonii genome is 155, 320 bp in length, containing a large single-copy region of 85, 280 bp, a small single-copy region of 17, 468 bp, a pair of inverted repeat regions of 26, 286 bp, and has a GC content of 37.8%. The genome encodes 113 unique genes, of which there are 79 protein-coding, 30 tRNA, and four rRNA genes. In addition, 18 genes contained introns, petB, petD, and rpl16 are coded for by two exons, and rps12 is identified as a trans-splicing gene. Phylogenetic analysis results strongly suggest that B. hamiltonii is closely related to B. marginatum . This study provides valuable genetic information to facilitate reliable identification.