Ethanol production from cassava starch by protoplast fusants of Wickerhamomyces anomalus and Galactomyces candidum. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Adeleye, Tolulope Modupe; Kareem, Sharafadeen Olateju; Bankole, Mobolaji O.; Atanda, Olusegun; Adeogun, Abideen I.
• Is Part Of: Egyptian journal of basic and applied sciences. Volume 7:Issue 1(2020)
• Page Start: 67
• Page End: 81
• Publication Date: 2020-01-01
• Subjects: Ethanol production -- protoplast fusion -- Wickerhamomyces anomalus -- Galactomyces candidum -- cassava starch fermentation; Science -- Periodicals; Technology -- Periodicals; Technology; Science; Science; Electronic journals; Periodicals; Periodical; 505
• DOI: 10.1080/2314808X.2020.1746884
• Languages: English
• ISSNs: 2314-808X

Abstract

ABSTRACT: The potential of protoplast fusants of Wickerhamomyces anomalus and Galactomyces candidum isolates for ethanol production from cassava starch was evaluated. The protoplasts were obtained from 18 h yeast cells digested by zymolase and fused by polyethylene glycol (PEG). Regenerated recombinants were randomly selected and examined for desired marker traits: rate of fermentation of glucose, maltose and ethanol tolerance. Cassava starch hydrolysates were obtained by the amylolytic action of partially purified amylase extracts from Aspergillus species . Three of the selected fusants exhibited improvements in fermentation parameters over the parents. Enhanced ethanol tolerance of (25% v/v) as against 20% of the parents was exhibited by fusants R4 and R7. All isolates displayed higher ethanol productivity than CO2 . The highest values of carbon dioxide productivity (4.79 L/L.h), volumetric ethanol productivity (11.54 g/L.h) and an ethanol mass concentration of 110.88 g/l were obtained from the fermentation by R1. Fusant R1 also had the highest fermentation efficiency (80%) and actual ethanol recovery (10.19%), as compared to other fusants and the parent yeast isolates. Parent isolates and selected fusants were characterized using molecular tools. The possibility of the occurrence of a genetic recombination in selected fusants as a result of protoplast fusion was observed.