Lactobacilli and bifidobacteria derived from infant intestines may activate macrophages and lead to different IL-10 secretion. Issue 12 (1st December 2020)

• Record Type: Journal Article
• Authors: Liang, Huijing; Luo, Zihao; Miao, Zhonghua; Shen, Xi; Li, Ming; Zhang, Xuguang; Chen, Jiehua; Ze, Xiaolei; Chen, Qiwei; He, Fang
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 84:Issue 12(2020)
• Page Start: 2558
• Page End: 2568
• Publication Date: 2020-12-01
• Subjects: Lactobacillus -- Bifidobacterium -- macrophages -- interleukin-10 -- signal pathway; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2020.1811948
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: In this study, three strains of lactobacilli and bifidobacteria originally isolated from healthy infants, were tested for their abilities to activate RAW264.7 cells. Gene expression and cytokine production of interleukin-10 (IL-10) of RAW264.7 cells were evaluated. The activation of extracellular regulated protein kinases 1/2 (ERK1/2), p38, and nuclear factor-κB (NK-κB) were also assessed. These results suggest lactobacilli and bifidobacteria in infants may promote production of IL-10 in macrophages, conferring a protective effect in hosts suffering from inflammation. Dimerization of TLR2 and MyD88 and subsequent phosphorylation of the key downstream signaling molecules, such as MAPKs and NK-κB, may be one of the key underlying mechanisms of activation of macrophages by these microbes. Bifidobacteria and lactobacilli induced macrophages to secrete IL-10 in a different manner, which may relate to their abilities to activate key signaling pathways mediated by TLR2 and MyD88. GRAPHICAL ABSTRACT: uf0001 Lactobacilli and bifidobacteria derived from infant intestines may activate macrophages and lead to different IL-10 secretion