Metabolic pathways of Chlorella sp. cells induced by exogenous spermidine against nitric oxide damage from coal-fired flue gas. (May 2021)

• Record Type: Journal Article
• Authors: Wang, Zhenyi; Cheng, Jun; Zhang, Xiangdong; Chen, Lechong; Liu, Jianzhong
• Is Part Of: Bioresource technology. Volume 328(2021)
• Publication Date: 2021-05
• Subjects: Nitric oxide -- Microalgae -- Spermidine -- Metabolomics -- Proteomics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.124827
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Spermidine induced NO fixation in amino acids and metabolites like 5-oxoproline. BC transport was enhanced by less NO reduced by spermidine. Spermidine protected Chlorella sp. from peroxidation damage caused by NO. Spermidine protected the photosynthetic system against NO stress. Exogenous spermidine increased biomass yield by 30.5% against NO. Abstract: To protect microalgae that are used for photosynthetic CO2 fixation against high NO concentrations from coal-fired flue gas, 500 μM exogenous spermidine was added into Chlorella sp. solution resulting in an elevation of biomass yield by 30.5% under 327 ppm NO. Metabolomics, proteomics and enzyme activities were analyzed, revealing three effects of spermidine on Chlorella sp. resistance to NO stress. First, spermidine induced NO fixation in amino acids and their metabolites, mainly in form of 5-oxoproline (1.51-fold), which occurred through intracellular conversion reactions between citrulline and arginine. Accordingly, cellular respiration was strengthened along with a weakened NO inhibition, which enhanced active transport with ATP consumption. Second, spermidine guarded Chlorella sp. against peroxidation damage by improving activity of antioxidant enzymes. Finally, it protected the photosynthetic system of Chlorella sp. by increasing abundance of related enzymes to enhance carbon fixation. Thus exogenous spermidine improved biomass production against NO environment.