Synthetic yeast genomes for studying chromosomal features. Issue 23 (October 2020)

• Record Type: Journal Article
• Authors: Jiang, Shuangying; Zhao, Shijun; Cai, Zelin; Tang, Yuanwei; Dai, Junbiao
• Is Part Of: Current opinion in systems biology. Issue 23(2020)
• Page Start: 1
• Page End: 7
• Publication Date: 2020-10
• Subjects: Synthetic genomes -- Chromosome number -- Ring chromosome -- Telomere -- rDNA -- tRNA genes -- Transposable elements -- Codons -- Introns; Systems biology -- Periodicals; 570
• DOI: 10.1016/j.coisb.2020.09.001
• Languages: English
• ISSNs: 2452-3100

Abstract

Abstract: De novo synthesis of a genome makes it possible to probe fundamental biological questions that are experimentally inaccessible with traditional approaches. For example, the functions of genomic features such as the repetitive nature of the ribosomal rRNA coding sequences, the pervasive presence of transposons, and the universal existence of introns and splicing machinery in eukaryotic systems were deliberately tested in the on-going Synthetic Yeast Genome Project (Sc2.0). In this review, we summarize these genomic features, which are analyzed through careful design in synthetic genomics projects up to now. Further reprogramming of these features and beyond could provide insights into corresponding biological processes in other organisms. Highlights: Various chromosomal features examined in Sc2.0 through careful design were summarized. Chromosomal features includes those at whole genome, within a chromosome or a gene. Reprogramming of synthetic chromosomes allows deeper understanding of genome biology.