Histone Deacetylase Is Required for GA-Induced Programmed Cell Death in Maize Aleurone Layers  . Issue 3 (29th September 2017)

• Record Type: Journal Article
• Authors: Hou, Haoli; Zheng, Xueke; Zhang, Hao; Yue, Mengxia; Hu, Yan; Zhou, Hong; Wang, Qing; Xie, Chengshen; Wang, Pu; Li, Lijia
• Is Part Of: Plant physiology. Volume 175:Issue 3(2017)
• Page Start: 1484
• Page End: 1496
• Publication Date: 2017-09-29
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.17.00953
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : GA induces PCD in maize aleurone layers through down-regulating the HAT activity and thereby indirectly increasing the relative HDAC activity, followed by the ROS-mediated signaling pathway. Abstract: Recent discoveries have shown that epigenetic regulation is an integral part of phytohormone-mediated processes. The phytohormone gibberellin (GA) triggers a series of events in cereal aleurone cells that lead to programmed cell death (PCD), but the signaling cascade mediating GA-induced PCD in cereal aleurone layers remains largely unknown. Here, we showed that histone deacetylase (HDAC) activity gradually increased relative to histone acetyltransferase (HAT) activity, leading to a global decrease in histone H3 and H4 acetylation levels during PCD of maize ( Zea mays ) embryoless aleurone layers after 3 d of treatment with GA. HDAC inhibition prevented GA-induced PCD in embryoless aleurone cells, whereas HAT inhibition resulted in PCD even in the absence of GA. Hydrogen peroxide concentrations increased in GA- or HAT inhibitor-treated aleurone cells due to reduced levels of reactive oxygen species scavengers. Hydrogen peroxide-treated aleurone cells showed no changes in the activity or expression of HATs and HDACs. We show that it is possible to predict whether epigenetic modification enzymes serve as a regulator of the GA-triggered PCD signaling pathway in maize aleurone layers. Taken together, these findings reveal that HDAC activity is required for GA-induced PCD in maize aleurone layers and regulates PCD via the reactive oxygen species-mediated signal transduction pathway.