The RING Finger Ubiquitin E3 Ligase OsHTAS Enhances Heat Tolerance by Promoting H2O2-Induced Stomatal Closure in Rice. Issue 1 (12th November 2015)

• Record Type: Journal Article
• Authors: Liu, Jianping; Zhang, Cuicui; Wei, Chuchu; Liu, Xin; Wang, Mugui; Yu, Feifei; Xie, Qi; Tu, Jumin
• Is Part Of: Plant physiology. Volume 170:Issue 1(2016)
• Page Start: 429
• Page End: 443
• Publication Date: 2015-11-12
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.15.00879
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : A RING finger ubiquitin ligase functions enhances heat tolerance through the modulation of H2 O2 -induced stomatal closure. Abstract: Heat stress often results in the generation of reactive oxygen species, such as hydrogen peroxide, which plays a vital role as a secondary messenger in the process of abscisic acid (ABA )-mediated stomatal closure. Here, we characterized the rice ( Oryza sativa ) HEAT TOLERANCE AT SEEDLING STAGE ( OsHTAS ) gene, which plays a positive role in heat tolerance at the seedling stage. OsHTAS encodes a ubiquitin ligase localized to the nucleus and cytoplasm. OsHTAS expression was detected in all tissues surveyed and peaked in leaf blade, in which the expression was concentrated in mesophyll cells. OsHTAS was responsive to multiple stresses and was strongly induced by exogenous ABA . In yeast two-hybrid assays, OsHTAS interacted with components of the ubiquitin/26S proteasome system and an isoform of rice ascorbate peroxidase. OsHTAS modulated hydrogen peroxide accumulation in shoots, altered the stomatal aperture status of rice leaves, and promoted ABA biosynthesis. The results suggested that the RING finger ubiquitin E3 ligase OsHTAS functions in leaf blade to enhance heat tolerance through modulation of hydrogen peroxide-induced stomatal closure and is involved in both ABA -dependent and DROUGHT AND SALT TOLERANCE-mediated pathways.