Astragalus Polysaccharides Inhibit Pancreatic Cancer Progression by Downregulation of TLR4/NF-κB Signaling Pathway. (12th July 2022)

• Record Type: Journal Article
• Authors: Fang, Jingjing; Wu, Zongyang; Wang, Jinbo; Feng, Jiye; Ying, Liping
• Other Names: Chen Xuncai Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2022(2022)
• Publication Date: 2022-07-12
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2022/5351611
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : Background . Pancreatic cancer (PC) leads to high human malignancy mortality worldwide. This study explored the role of Astragalus polysaccharide (APS) on human PC PANC-1 cells and its underlying mechanisms. Method . Cell viability, proliferation, apoptosis, invasion, and migration were measured by CCK-8, EdU incorporation, flow cytometry, Transwell, and wound healing assay, respectively. ELISA assay was utilized to detect the IL-1 α, IL-4, IL-6, IL-8, and TNF- α levels. The western blot assay was performed to measure the level changes of cell function-related proteins. The transportation of NF- κ B P65 protein was detected through immunofluorescence assay. Results . Compared with the control group, APS treatment could significantly inhibit cell proliferation. APS treatment could also suppress cell migration and invasion ability and induce apoptosis and inflammation in PANC-1 cells. Furthermore, APS inhibited the activation of TLR4/NF- κ B signaling pathway via suppressing the phosphorylation and transportation of NF- κ B P65 into the PANC-1 cell nucleus. Conclusion . APS suppresses PANC-1 cell viability, proliferation, migration, and invasion while inducing inflammation and apoptosis. APS might regulate PC cell motility via downregulating TLR4/NF- κ B signaling pathway.