TPPU Downregulates Oxidative Stress Damage and Induces BDNF Expression in PC-12 Cells. (13th July 2022)

• Record Type: Journal Article
• Authors: Wu, Qiong; Lin, Minlin; Wu, Peng; Zhao, Chongyan; Yang, Shuang; Yu, Haiying; Xian, Wenjiao; Song, Jingfang
• Other Names: Chen Gang Academic Editor.
• Is Part Of: Computational and mathematical methods in medicine. Volume 2022(2022)
• Publication Date: 2022-07-13
• Subjects: Medicine -- Computer simulation -- Periodicals; Medicine -- Mathematical models -- Periodicals; 610.11
• DOI: 10.1155/2022/7083022
• Languages: English
• ISSNs: 1748-670X

Abstract

Abstract : Objective . Ischemia-reperfusion is an ongoing clinical challenge that can lead to a series of pathological changes including oxidative stress. The inhibition of soluble epoxide hydrolase inhibitor (sEH) by 1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy)phenyl]urea (TPPU) results in an anti-inflammatory, cardioprotective, and blood vessel growth-promoting effects. Therefore, this study focused on the protective effect of TPPU on a rat pheochromocytoma (PC-12) cell oxidative stress model induced by H2 O2 . Methods . CCK-8 and Hoechst 33342 were used to evaluate cell apoptosis and western blot to detect the apoptotic proteins and brain-derived neurotrophic factor (BDNF) expression. Result . The incubation with 100 μ M, 50 μ M, and 25 μ M TPPU significantly increased PC-12 cell viability. Epoxyeicosatrienoic acid (EET) pretreatment also protected PC-12 cells from oxidative stress. In addition, TPPU reduced caspase-3 and Bax expression and induced Bcl-2 expression, and EETs exerted the same effect on caspase-3 expression as TPPU. A positive relationship was found between TPPU or EET incubation and BDNF expression. Conclusion . These results revealed that TPPU reduced PC-12 cell oxidative stress injury induced by H2 O2 and promoted BDNF expression.