Tea polysaccharides from Camellia sinensis: chemical analysis, structural characterization, and inhibition of HeLa cells activity. Issue 3 (3rd April 2021)

• Record Type: Journal Article
• Authors: Gao, Yuefang; Wang, Yancheng; Ji, Xuening; Xiao, Yao; Xiao, Bin; Peng, Pai
• Is Part Of: International journal of polymer analysis and characterization. Volume 26:Issue 3(2021)
• Page Start: 240
• Page End: 252
• Publication Date: 2021-04-03
• Subjects: Tea polysaccharides -- hydrothermal extraction -- chemical analysis -- structural characterization -- HeLa cells; Polymers -- Analysis -- Periodicals; 547.705
• DOI: 10.1080/1023666X.2021.1877957
• Languages: English
• ISSNs: 1023-666X

Abstract

Abstract: Tea polysaccharides (TPS) were analyzed and characterized by HPAEC, GPC, FT-IR, AFM, HSQC, and HMBC NMR as well as methylation. The TPS mainly consisted of neutral (F0, F0.2, and F0.3 ) and acidic polysaccharides (F0.1 ) rich in glucuronic acid. Moreover, anti-proliferative activity of the TPS on HeLa cells was investigated. It was found that F0 exhibited the highest inhibition rate (36.7%) with 200 µg/ml treatment for 48 h. Structural characterization revealed that F0 had a backbone chain of (1→4)- β -d -Glc p, (1→6)- α -d -Gal p, (1→2)- α -l -Rha p and (1→2, 4)- α -l -Rha p residues with (1→6)- α -d -Gal p and acetyl group side chains.