BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Verma, Sonam; Kang, Amandeep Kaur; Pal, Rahul; Gupta, Satish Kumar
• Is Part Of: Cell adhesion & migration. Volume 14:Issue 1(2020)
• Page Start: 24
• Page End: 41
• Publication Date: 2020-01-01
• Subjects: IFN-γ -- BST2 -- trophoblast invasion -- E-cadherin -- STAT1 -- AKT; Cell adhesion -- Periodicals; Cell migration -- Periodicals; Cell interaction -- Periodicals; 574.876
• DOI: 10.1080/19336918.2019.1710024
• Languages: English
• ISSNs: 1933-6918

Abstract

ABSTRACT: The mechanism by which interferon-gamma (IFN-γ) downregulates trophoblast invasion needs further investigation. Treatment of HTR-8/SVneo cells with IFN-γ led to a decrease in their invasion concomitant with an increased expression of BST2. Silencing of BST2 by siRNA showed a significant increase in their invasion and spreading after treatment with IFN-γ as well as downregulated expression of E-cadherin. Further, STAT1 silencing inhibited the IFN-γ-dependent increase in the expression of BST2 and E-cadherin. Treatment of HTR-8/SVneo cells with IFN-γ led to the activation of AKT, and its inhibition with PI3K inhibitor abrogated IFN-γ-mediated decrease in invasion/spreading and downregulated BST2 and E-cadherin expression. Collectively, IFN-γ decreases the invasion of HTR-8/SVneo cells by STAT1 and AKT activation via increased expression of BST2 and E-cadherin.