Naturally prefabricated 3D chitinous skeletal scaffold of marine demosponge origin, biomineralized ex vivo as a functional biomaterial. (1st January 2022)

• Record Type: Journal Article
• Authors: Machałowski, Tomasz; Idaszek, Joanna; Chlanda, Adrian; Heljak, Marcin; Piasecki, Adam; Święszkowski, Wojciech; Jesionowski, Teofil
• Is Part Of: Carbohydrate polymers. Volume 275(2022)
• Publication Date: 2022-01-01
• Subjects: Biopolymers -- Chitin -- Biomineralization -- Human cells -- Tissue engineering; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118750
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Solutions developed by nature for structural and functional optimization of three-dimensional (3D) skeletal structures provide unique windows not only into the evolutionary pathways of organisms, but also into bioinspired materials science and biomimetics. Great examples are naturally formed 3D chitinous scaffolds of marine sponge remain a focus of modern biomedicine and tissue engineering. Due to its properties like renewability, bioactivity, and biodegradability such constructs became very interesting players as components of organic-inorganic biocomposites. Herein, we developed chitin-based biocomposites by biomimetic ex vivo deposition of calcium carbonate particles using hemolymph from the cultivated mollusk Cornu aspersum and chitinous matrix from the marine demosponge Aplysina fistularis . The biological potential of the developed biofunctionalized scaffolds for bone tissue engineering was evaluated by investigating the spreading and viability of a human fetal osteoblast cell line has been determined for the first time. Performed analyses like dynamic mechanical analysis and atomic force microscopy shown that biofunctionalized scaffold possess about 4 times higher mechanical resistance. Moreover, several topographical changes have been observed, as e.g., surface roughness (Rq) increased from 31.75 ± 2.7 nm to 120.7 ± 0.3 nm. The results are indicating its potential for use in the modification of cell delivery systems in future biomedical applications. Highlights: 3D chitinous skeleton was isolated from marine sponge Aplysina fistularis by chemical acid-base treatment. Ex-vivo mimicking Cornu aspersum mollusk shell regeneration process allow growth bioCaCO3 particles onto scaffold surface. Macro- and nanoscale mechanical and biophysical properties of the scaffolds were studied. Biofunctionalization improved human fetal osteoblast spreading and viability.