MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5. (5th July 2022)

• Record Type: Journal Article
• Authors: Gong, Fan; Li, Xiaoliang; Zhang, Hanling; Wu, Jianke; Ma, Guoxu; Zhang, Bowen; Gao, Jian; Ding, Yi; Huang, Yonglu; Xia, Kun; Cheng, Suoli; Zhou, Xuebing; Shi, Jiandang; Zhao, Fei
• Other Names: Chen Gang Academic Editor.
• Is Part Of: Computational and mathematical methods in medicine. Volume 2022(2022)
• Publication Date: 2022-07-05
• Subjects: Medicine -- Computer simulation -- Periodicals; Medicine -- Mathematical models -- Periodicals; 610.11
• DOI: 10.1155/2022/6481846
• Languages: English
• ISSNs: 1748-670X

Abstract

Abstract : Objective. To explore the effect of microRNA (miR)-192-5p on the inflammatory and fibrotic responses of tendon cells. Methods. Tendon cells were treated with transforming growth factor- β 1 (TGF- β 1). The expression of miR-192-5p and nuclear factor of activated T cells 5 (NFAT5) in tendon cells were detected by RT-qPCR. The expressions of inflammatory and fibrosis-related factors were detected by RT-qPCR and Western blot. MiR-192-5p binds to NFAT5 targeting by TargetScan and dual-luciferase reporter gene assay. The expression of the NFAT5 gene was detected by RT-qPCR and Western blot. Detection of apoptosis in tendon cells by flow cytometry. Results. MiR-192-5p was downregulated in tendon cells, and the expression level gradually decreased with the prolong of TGF- β 1 treatment. The expression of NFAT5 increased with the treatment time of TGF- β 1. The expression of miR-192-5p decreased collagen III (COLIII), α smooth muscle actin ( α -SMA), matrix metalloproteinase- (MMP-) 1, and MMP-8 expression, thereby inhibiting TGF- β 1-induced fibrosis in tendon cells. The expression of miR-192-5p decreased the expression of tumor necrosis factor- α (TNF- α ), interleukin (IL)-6, and IL-1 β, thereby alleviating TGF- β 1-induced inflammatory response and reduce apoptosis in tendon cells. NFAT5 is a direct target of miR-192-5p in tendon cells. The upregulation of NFAT5 reversed the effect of miR-192-5p on the fibrotic activity and inflammatory response of TGF- β 1-stimulated tendon cells. Conclusions. MiR-192-5p alleviates fibrosis and inflammatory responses of tendon cells by targeting NFAT5.