A systematic investigation on spectroscopic, conformational, and interactional properties of polypeptide/nanomaterial complex: effects of bio-based synthesized maghemite nanocomposites on human serum albumin. Issue 4 (1st October 2020)

• Record Type: Journal Article
• Authors: Behzad, Farahnaz; Jafarirad, Saeed; Samadi, Azam; Barzegar, Abolfazl
• Is Part Of: Soft materials. Volume 18:Issue 4(2020)
• Page Start: 471
• Page End: 486
• Publication Date: 2020-10-01
• Subjects: Spectroscopic analysis -- maghemite nanocomposites -- Fe2O3/rGO nanocomposites -- human serum albumin -- natural polymers -- zeta potential; Materials science -- Periodicals; 620.11
• DOI: 10.1080/1539445X.2020.1716800
• Languages: English
• ISSNs: 1539-445X

Abstract

ABSTRACT: The aim of this study was to prepare novel bio-based maghemite (Fe2 O3 ) nanocomposites as reduced graphene oxide (rGO)-doped Fe2 O3 nanocomposites (Fe2 O3 /rGO NCs) and to investigate on their interaction with human serum albumin (HSA) as a natural polymer. Various photochemical and conformational techniques such as Fourier transform infrared spectrum, X-ray diffraction, Raman spectroscopy, scanning electron microscopes/energy-dispersive X-ray, circular dichroism, synchronous fluorescence spectroscopy, and resonance light scattering were used for characterizing the Fe2 O3 /rGO NCs and studying their interaction with HSA. The spectroscopic studies indicated that a complex formed between HSA and bio-based Fe2 O3 /rGO NCs induced structural changes on HSA. The helicity of HSA was decreased and the random coil and β-turn in the secondary structure of HSA were enhanced. In addition, the analysis of fluorescence spectra showed that Fe2 O3 /rGO NCs had a strong ability to quench the intrinsic fluorescence of HSA by static quenching mechanisms. It has been claimed that the bio-based production of nanomaterials possess no remarkable un-favored effects in biomedical fields of applications. However, the obtained results of our study demonstrate the deep influence of bio-based Fe2 O3 /rGO NCs on HSA.