Steroid androgen 17 alpha methyltestosterone used in fish farming induces biochemical alterations in zebrafish adults. Issue 11 (18th July 2020)

• Record Type: Journal Article
• Authors: Rivero-Wendt, Carla Letícia Gediel; Miranda-Vilela, Ana Luisa; Domingues, Inês; Oliveira, Rhaul; Monteiro, Marta Sofia; Moura-Mello, Monica A. M.; Matias, Rosemary; Soares, Amadeu Mortágua Velho Maia; Grisolia, Cesar Koppe
• Is Part Of: Journal of environmental science and health. Volume 55:Issue 11(2020)
• Page Start: 1321
• Page End: 1332
• Publication Date: 2020-07-18
• Subjects: Glutathione-S-transferase -- micronucleus -- comet assay -- biochemical markers -- methyltestosterone; Environmental engineering -- Periodicals; Environmental sciences -- Periodicals; Ecology -- periodicals; Hazardous Substances -- periodicals; 628
• DOI: 10.1080/10934529.2020.1790954
• Languages: English
• ISSNs: 1093-4529

Abstract

Abstract: The 17 alpha methyltestosterone (MT) hormone is fed to Oreochromis niloticus larvae in fish farms with the purpose of inducing sex reversal. The aim of this study was to evaluate the toxicity and sub-lethality of MT (99.9% purity) and cMT (a commercial MT with 90% purity) in zebrafish ( Danio rerio ) adults, where the animals were exposed to concentrations of 0, 4, 23, 139, 833 and 5000 µg/L for 96 hours. Genotoxicity was evaluated by micronucleus test (MN), nuclear abnormalities (NA) and comet assay. A low genotoxic potential of MT was showed, inducing micronucleus, nuclear abnormalities and DNA damage in Danio rerio, depending on the use of MT or cMT, gender and tested concentrations. In the sub-lethality trials, there was a basal difference in the activity of the enzymatic biochemical markers for males and females, while the Glutatione S transferase (GST) activity decreased in all analyzed tissues, and for males the enzymatic activity decreased only in the intestine. Results suggest that MT has a toxic potential to fish because it alters enzymatic metabolic pathways and may pose a risk to the ecosystems.