False-Positive Clostridium difficile in Negative-Control Reactions Peak and Then Decrease with Repetitive Refrigeration of Immunoassay. (11th September 2014)

• Record Type: Journal Article
• Authors: Rodriguez-Palacios, Alexander; Stämpfli, Henry R.; Chang, Yung-Fu
• Other Names: Gómez Isabel Academic Editor.
• Is Part Of: ISRN obstetrics and gynecology. Volume 2014(2014)
• Publication Date: 2014-09-11
• Subjects: Obstetrics -- Periodicals; Gynecology -- Periodicals; Pregnancy Complications; Genital Diseases, Female; Gynecology; Obstetrics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 618.2
• DOI: 10.1155/2014/128120
• Languages: English
• ISSNs: 2090-4436

Abstract

Abstract : Aberrant false-positive reactions in negative-controls during ELISA testing for Clostridium difficile indicated the potential for false-diagnoses. Experiments with 96-well products showed a maximum peak of false-positive immunoassay reactions with the provided negative-control reagents after 5 refrigeration-to-room temperature cycles (P < 0.001 ), decreasing thereafter with additional refrigeration cycles. Because repetitive refrigeration causes a peak of false-positives, the use of single negative-controls per ELISA run might be insufficient to monitor aberrant preanalytical false-positives if immunoassays are subject to repetitive refrigeration.